Isolating Auxotrophic Mutants in Aspergillus nidulans
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چکیده
A method for the selection of auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin has been developed. The method results in about 9000fold enrichment. All types of auxotrophs were obtained. Under certain conditions preferential selection of nucleic acid hydrolysate-requiring auxotrophs was observed. The frequency of occurrence of these mutants was about 50 % of all colonies tested. Cysteine auxotrophs of A . nidulans were obtained for the first time by means of this method.
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